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Chapters
- 00:05Title
- 01:39Combine Lipids and Create a Lipid Film
- 02:12Prepare Liposome Solution
- 03:08Extrusion and Purification of Liposomes
- 04:30Test Column Fractions
- 05:33BCL-2 Family Studies Using LUVs
- 06:11Results: BCL-2 Regulation of LUV Permeabilization
- 07:49Conclusion
Biochemically-defined large unilamellar vesicles (LUVs) are a convenient model system to analyze BCL-2 family interactions with immediate implications in better understanding the mitochondrial pathway of apoptosis. A method to produce LUVs, along with standard BCL-2 family protein combinations and controls to examine LUV permeabilization, are presented.
