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Chapters
- 00:05Title
- 01:50Mass Spectrometry Measurement 1: Proton-bound Cluster Ion Formation
- 04:05Mass Spectrometry Measurement 2: the Collosion-induced Dissociation (CID) Bracketing Experiments
- 05:36Mass Spectrometry Measurement 3: the Kinetic Method
- 06:51Data Analysis
- 08:32Results: CID Spectra and Thermo-kinetic Plots
- 09:56Conclusion
The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.
Tags
Gas-phase AciditiesOligopeptidesAmino Acid ResiduesFolded ProteinsCysteine ResidueN-terminusC-terminusExperimental StudiesCondensed PhaseAqueous SolutionsSolvent EffectsActive SitesInterior RegionIntrinsic Acid-base PropertiesSolvent-free EnvironmentMeasurement MethodCooks Kinetic MethodMass SpectrometerElectrospray Ionization (ESI) Ion Source
