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Chapters
- 00:05Title
- 01:50Mass Spectrometry Measurement 1: Proton-bound Cluster Ion Formation
- 04:05Mass Spectrometry Measurement 2: the Collosion-induced Dissociation (CID) Bracketing Experiments
- 05:36Mass Spectrometry Measurement 3: the Kinetic Method
- 06:51Data Analysis
- 08:32Results: CID Spectra and Thermo-kinetic Plots
- 09:56Conclusion
Se describe la determinación de la acidez de la fase de gas de oligopéptidos que contienen cisteína. Los experimentos se realizaron con un espectrómetro de masas de triple cuadrupolo. La acidez relativa de los péptidos se miden utilizando experimentos de disociación inducida por colisión, y la acidez cuantitativas se determinaron utilizando los cocineros extendidas método cinético.
Tags
Gas-phase AciditiesOligopeptidesAmino Acid ResiduesFolded ProteinsCysteine ResidueN-terminusC-terminusExperimental StudiesCondensed PhaseAqueous SolutionsSolvent EffectsActive SitesInterior RegionIntrinsic Acid-base PropertiesSolvent-free EnvironmentMeasurement MethodCooks Kinetic MethodMass SpectrometerElectrospray Ionization (ESI) Ion Source
