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In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 02:12Methods-Biocontainment Facility
- 02:47Methods-Animal Preparation
- 03:28Methods-Intranasal Infection
- 03:49Methods-Luciferin Injection
- 04:10Preparation for Imaging
- 05:09LivingImage Software Imaging
- 05:39Image Reconstruction
- 06:083-Dimensional Reconstruction
- 09:29Recovery and Cleanup
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study.
Tags
In Vivo Imaging Systems (IVIS)Neuro-invasive Encephalitic VirusImaging TechnologyViral ResearchAnimal ModelsScientific AccuracyReduction Of Animal UsageStatistical AccuracyVaccine Efficacy StudiesMorbidity And Mortality EndpointsIn Vivo Imaging Systems (IVIS)Firefly Bioluminescent EnzymeCentral Nervous System (CNS)Encephalitic VirusMurine ModelIntranasal InfectionTC83-LucAttenuated Venezuelan Equine Encephalitis VirusLuciferase Gene
