Journal
/
생물학
/
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
JoVE 신문
생물학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

DOI:
10.3791/50102-v

•

15:33 min

•

August 04, 2013

•

, , , ,
1Departamento de Biología Molecular,Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 2Servicio de Citometría de Flujo y Clasificación Celular (SECIF),Instituto de Investigaciones Biológicas Clemente Estable, 3Sección Bioquímica, Facultad de Ciencias,Universidad de la República

Chapters

  • 00:00Title
  • 02:02Preparation of the Cellular Suspension
  • 04:55Flow Cytometric Analysis
  • 08:29Results
  • 14:22Conclusions

Summary

자동 번역

자동 번역

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

Tags

Testicular Cell SuspensionMammalian SpermatogenesisCell EnrichmentCell PurificationMechanical DisaggregationFlow CytometryGene Expression Studies

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/50102?article_type=v

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
check_url/kr/50102?article_type=v

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
check_url/kr/50102?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/kr/50102?article_type=v

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
check_url/kr/50102?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/kr/50102?article_type=v

Preparation of Quality Inositol Pyrophosphates
check_url/kr/50102?article_type=v

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
check_url/kr/50102?article_type=v

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
check_url/kr/50102?article_type=v

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
check_url/kr/50102?article_type=v

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Read Article