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Bioengineering

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Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
 

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy

Article DOI: 10.3791/50163-v 10:24 min February 21st, 2013
February 21st, 2013

챕터

요약

The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.

Tags

Keywords: Endothelial Glycocalyx Atomic Force Microscopy Mechanical Properties Leukocyte Adhesion Endothelial Cell Stiffness Glycocalyx Modulus Glycocalyx Thickness Cell Surface Topography
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