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Chapters
- 00:05Title
- 02:05Wafer Fabrication
- 04:17Polydimethylsiloxane Chip Fabrication
- 06:50Experimental Setup
- 08:34Seeding of Bacterial Cells and Time-lapse Imaging
- 10:30Results: Quantification of Microcolony Growth
- 11:26Conclusion
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
