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JoVE Journal
Bioengineering

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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis
 
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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Article DOI: 10.3791/50560-v 12:04 min December 6th, 2013
December 6th, 2013

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In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

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Keywords: Microfluidic Picoliter Bioreactor Single-cell Analysis Fabrication System Setup Operation Polydimethylsiloxane SU-8 Photolithography Corynebacterium Glutamicum Escherichia Coli Single-cell Heterogeneity Biotechnology Cell Cultivation Microbial Culture Time-lapse Microscopy
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