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Localizzazione e quantificazione relativa dei nanotubi di carbonio in cellule con immagini multispettrali Citometria a Flusso
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
DOI:

14:09 min

December 12, 2013

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Chapters

  • 00:05Title
  • 01:38Preparation of Water Dispersible Carbon Nanotubes
  • 03:24ImageStream Acquisition
  • 04:07Post Processing: IDEAS Analysis Software (Version 4.0)
  • 08:52Results: Representative Analyses of CNT Internalization
  • 13:37Conclusion

Summary

자동 번역

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

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