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The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells
 

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Article DOI: 10.3791/50610-v • 09:33 min • September 20th, 2013
September 20th, 2013 •
1,2, 1, 1, 3, 1
1Laboratory of Molecular Biology of the Cell, Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille, Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer, The Rockefeller University

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요약
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The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for studies of retinal degeneration and neurodegenerative diseases or photoreceptor cell development.

Tags

Tomato/GFP-FLP/FRT Method Live Imaging Mosaic Adult Drosophila Photoreceptor Cells Clonal Analysis Genetic Screens Signaling Pathways Eye Development Neuronal Degeneration Cornea Neutralization Technique Flipase-mediated Recombination High-throughput Screening PR Survival And Function Kinetic Analyses PR Degeneration Specific Genes Cell Autonomy Issues Planar Cell Polarity
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