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JoVE Journal Chemistry
Isolatie en chemische karakterisering van Lipid A van Gram-negatieve bacteriën
 
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Isolatie en chemische karakterisering van Lipid A van Gram-negatieve bacteriën

Article DOI: 10.3791/50623-v • 12:57 min • September 16th, 2013
September 16th, 2013 •
1, 2, 2, 1,3
1Section of Molecular Genetics and Microbiology, The University of Texas at Austin, 2Department of Chemistry and Biochemistry, The University of Texas at Austin, 3The Institute of Cellular and Molecular Biology, The University of Texas at Austin

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Isolatie en karakterisering van het lipide Een domein van lipopolysaccharide (LPS) van gram-negatieve bacteriën geeft inzicht in celoppervlak gebaseerde mechanismen van resistentie tegen antibiotica, bacteriële overleving en fitness, en hoe chemisch diverse lipide Een moleculaire species verschillend moduleren gastheer aangeboren immuunrespons.

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Chemie membraanlipiden Toll-like receptoren endotoxinen Glycolipiden Lipopolysacchariden lipide A Microbiologie lipiden lipide A Bligh-Dyer dunne laag chromatografie (TLC) lipopolysacharide massaspectrometrie botsingsgeïnduceerde fragmentatie (CID ) Photodissociation (PD)
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