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Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
 

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

Article DOI: 10.3791/50627-v • 11:45 min • May 26th, 2015
May 26th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute

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요약
Automatic Translation

Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

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Generation 3-dimensional Quantitation Arterial Lesions Mice Optical Projection Tomography Cardiovascular Disease Research Lesion Formation Novel Therapies Atherosclerosis-prone Mice Surgical Methods Lesion Induction Dietary Modification Histological Techniques Non-destructive Analysis Immunohistochemical Analyses Neointimal Lesions Wire-insertion Ligation Femoral Artery Atherosclerotic Lesions Atherogenic Diet ApoE-deficient Mice OPT Imaging Autofluorescent Emission Histological Analysis Immunohistochemical Analysis Lesion Size Calculation
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