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JoVE Journal
Immunology and Infection

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Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
 

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

Article DOI: 10.3791/50667-v 07:07 min September 12th, 2013
September 12th, 2013

챕터

요약

The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.

Tags

Keywords: Respiratory Burst Innate Immunity Zebrafish Reactive Oxygen Species Phagocyte Fluorometric Assay Microplate Reader Fluorogenic Substrate Developmental Stage Kidney Fish Species
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