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1The Markey Cancer Center,University of Kentucky College of Medicine, 2Graduate Center for Toxicology,University of Kentucky College of Medicine, 3Department of Molecular and Biomedical Pharmacology,University of Kentucky College of Medicine, 4Department of Pediatrics,University of Kentucky College of Medicine
Chapters
- 00:05Title
- 02:12Preparation of C57Bl/6 K14-Scf Mice and Topical Forskolin Treatment
- 04:31Reflective Colorimetry and Calculating the Minimal Erythematous Dose
- 08:15Representative Melanin Induction Results
- 11:43Conclusion
Epidermal melanin is induced by topical application of forskolin in a murine model of the fair-skinned UV-sensitive human. Pharmacologic manipulation of cAMP levels in the skin and epidermal darkening strongly protect against UV-mediated inflammation (sunburn) as measured by the minimum erythematous dose (MED) assay.
