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从救援的cDNA重组新城疫病毒
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Rescue of Recombinant Newcastle Disease Virus from cDNA
DOI:

10:55 min

October 11, 2013

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Chapters

  • 00:05Title
  • 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
  • 02:40Transfection of Mammalian Cells
  • 03:48Co-culture of Mammalian Cells with Avian Cells
  • 05:22Infection of Chicken Embryonated Eggs
  • 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
  • 07:52Hemagglutination (HA) Assay
  • 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
  • 10:33Conclusion

Summary

자동 번역

新城疫病毒(NDV)已被广泛研究,在过去的几年里,以开发新的载体接种疫苗和治疗,等等。这些研究已经可能由于技术由cDNA拯救重组病毒,如那些我们在这里描述。

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