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Whole-cell MALDI-TOF massaspectrometrie is een nauwkeurige en snelle methode om verschillende vormen van macrofaagactivering Analyseer
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation
DOI:

11:25 min

December 26, 2013

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Chapters

  • 00:05Title
  • 01:39Preparation of Human CD14+ Monocytes
  • 03:44Differentiation of Monocytes into Macrophages
  • 04:25Preparation of Biological Samples for MALDI-TOF MS
  • 05:08Whole Cell MALDI-TOF MS Analysis
  • 07:22Bioinformatic Analysis
  • 08:05Results: Macrophage Responsiveness
  • 10:56Conclusion

Summary

자동 번역

This protocol describes the use of whole-cell MALDI-TOF mass spectrometry on eukaryotic cells. Here, we illustrate the accuracy of this technique by analyzing the multiple activation states of macrophages in response to their microenvironment.

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