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Chapters
- 00:05Title
- 01:18Identifying TALEN Target Sites
- 02:30Embryo Isolation
- 04:23Embryo Microinjection
- 06:33Embryo Transfer
- 09:15Identifying Founders with Targeted Mutations
- 11:21Conclusion
Designer nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to modify the genome of mouse preimplantation embryos by triggering both the nonhomologous end joining (NHEJ) and homologous recombination (HR) pathways. These advances enable the rapid generation of mice with precise genetic modifications.
