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Chapters
- 00:05Title
- 02:47Construction of PXR and SRC-1 Fusions in Yeast Vectors
- 03:49Yeast Two-hybrid Assays
- 05:26X-gal Filter Assay
- 06:32Liquid β-gal Assay
- 07:49Results: X-gal Lift and β-gal Liquid Assay Interpretation
- 09:06Conclusion
Ketoconazole binds to and antagonizes Pregnane X Receptor (PXR) activation. Yeast high throughput screens of PXR mutants define a unique region for ketoconazole binding. This yeast-based genetic method discovers novel nuclear receptor interactions with ligands that associate with surface binding sites.
