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用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
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用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

DOI:
10.3791/51114-v

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15:28 min

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January 17, 2014

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, , , , , , , ,
1Inserm U1019 – CNRS UMR 8024, Institut Pasteur de Lille,Université de Lille

Chapters

  • 00:05Title
  • 01:44Preparation of the Bacterial Suspension
  • 03:07High-throughput Genome-wide siRNA Screening
  • 06:16High-throughput Compound Screening
  • 08:27Image Acquisition and Analysis
  • 11:10Results: Mtb Colonization in Host Cells is Inhibited by Silencing Dectin-1 and by Isonazid or Rifampicin Treatment
  • 14:37Conclusion

Summary

자동 번역

자동 번역

在这里，我们描述了一种表型检测，适用于小干扰合成RNA（siRNA）、化合物和 结核 分枝杆菌突变库的高通量/高含量屏幕。该方法依靠使用自动共聚焦显微镜检测荧光标记的荧光结 核分枝杆菌 。

Tags

TuberculosisMycobacteriaIntracellularPhenotypic AssayHigh-throughput ScreeningHigh-content ScreeningMycobacterium TuberculosisDrug ResistanceGenetic ScreensChemical ScreensFluorescence MicroscopyImage AnalysisHost-pathogen Interaction

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