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Chapters
- 00:05Title
- 01:22Culturing Mouse Embryonic Fibroblasts (MEFs)
- 04:17Culturing Neurons
- 06:22Visualizing G3BP-containing Stress Granule Assembly and Dynamics
- 08:39Stress Granule Formation in MEFs and Murine Hippocampal Neurons
- 09:38Conclusion
Stress granules (SGs) are cytoplasmic RNA granules containing stalled ribonucleoprotein particles (RNPs), and important in cellular response to various stresses. Dynamics of SGs can be followed in live cells by visualizing the localization of a tagged component of SGs in transfected primary cells after stress.
