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JoVE Journal
Immunology and Infection

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Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
 

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Article DOI: 10.3791/51207-v 08:11 min September 1st, 2015
September 1st, 2015

챕터

요약

Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.

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Keywords: HIV-1 CD4+ T Cells Plasmacytoid Dendritic Cells Ex Vivo Co-culture Innate Sensing Type-I Interferon Flow Cytometry PBMC TLR Agonist ISGF3 Pathway
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