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Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
 

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Article DOI: 10.3791/51248-v • 14:14 min • February 28th, 2014
February 28th, 2014 •
1,2, 3, 3,4, 1,2,4,5
1London Regional Cancer Program, London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry, Western University, 3Special Hematology/Flow Cytometry, London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology, Western University

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요약
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Circulating tumor cells (CTCs) are prognostic in several metastatic cancers. This manuscript describes the gold standard CellSearch system (CSS) CTC enumeration platform and highlights common misclassification errors. In addition, two adapted protocols are described for user-defined marker characterization of CTCs and CTC enumeration in preclinical mouse models of metastasis using this technology.

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Keywords: Semiautomated CTC Assay Circulating Tumor Cells CellSearch System CTC Detection CTC Enumeration CTC Characterization CTC Capture Preclinical Mouse Models Metastasis CTC Misclassification
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