/

의학

/

癌症干细胞从人类前列腺癌样本隔离

JoVE 신문
의학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 의학

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Please note that all translations are automatically generated. Click here for the English version.

癌症干细胞从人类前列腺癌样本隔离

DOI:

10.3791/51332-v

•

07:16 min

•

March 14, 2014

•

Samuel J. Vidal, S. Aidan Quinn, Janis de la Iglesia-Vicente, Dennis M. Bonal, Veronica Rodriguez-Bravo, Adolfo Firpo-Betancourt, Carlos Cordon-Cardo, Josep Domingo-Domenech

¹Department of Pathology,Icahn School of Medicine at Mount Sinai, ²Molecular Biology Program,Memorial Sloan-Kettering Cancer Center

Chapters

00:05Title
01:17Harvesting and Processing of Human Prostrate Cancer Tissue
02:18Generation of Cell Suspensions from Tissue Sections
04:07Isolation of Prostrate Cancer Stem Cells (CSCs) by FACS
05:56Results: Representative Human Prostrate Cancer Cell Populations
06:50Conclusion

Summary

자동 번역

肿瘤干细胞（肿瘤干细胞）直接从人体组织中的隔离是必要的其生物学特性。这个手稿描述了从人体组织前列腺肿瘤干细胞的分离的方法，同时还提供疑难排解困难的步骤提示。

Tags

Keywords: Cancer Stem Cells Prostate Cancer HLA Class I Flow Cytometry Human Tumor Samples Cell Isolation Molecular Characterization Functional Validation Immunodeficient Mice

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

使用实时活体成像在肿瘤纳米粒子吸收的评价

人牙髓干细胞的分离，鉴定和比较性恒牙使用两种不同的方法，得出

延时成像的主要癌前乳腺上皮细胞来源于遗传工程小鼠乳腺癌模型

来自患者的细胞培养和分离的CD133<sup> +</sup>假定的肿瘤干细胞的恶性黑色素瘤

人类前列腺上皮细胞形成的使用鼠类泌尿生殖窦间质细胞和人类干细胞的组织重组

人类前列腺癌转移的原位小鼠模型

丰富的人力细胞系化疗耐药性卵巢癌干细胞

研究胰腺癌干细胞特性开发新的治疗策略

从小鼠癌症模型的循环肿瘤细胞的分离和繁殖

在前列腺癌的临床组织中miRNA的表达分析

Read Article