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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
 

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Article DOI: 10.3791/51426-v • 10:26 min • March 11th, 2014
March 11th, 2014 •
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1Advanced Cell Diagnostics, Inc.

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요약
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The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

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RNAscope In Situ Detection Transcriptionally Active Human Papillomavirus Head And Neck Squamous Cell Carcinoma Oncogenic HPV E6/E7 MRNA Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) RNA Extraction Tumor Tissue Context Morphological Correlation Routine Clinical Practice RNA In Situ Hybridization Technology Formalin-fixed Paraffin-embedded (FFPE) Tissue Single Molecule Sensitivity Single Cell Resolution RNA Biomarker HPV Assay High-risk HPV Genotypes Sensitivity And Specificity Gold Standard Method Prognostic Clinical Outcome Oropharyngeal Cancer Patients
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