JoVE Journal
Biology
Biology
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.