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Chapters
- 00:05Title
- 02:01Preparing Samples and Performing the Experiment
- 06:47Data Analysis
- 09:24Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry
- 12:29Conclusion
Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.
