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Chapters
- 00:05Title
- 01:15Converting Human Pluripotent Stem Cell (hPSC) Colonies on Feeders to Noncolony Type Monolayers (NCM)
- 04:41NCM Culture for Transfection of MicroRNAs
- 05:51Results: Representative NCM Culture Analyses
- 07:48Conclusion
Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.
