JoVE Journal
Biology
Biology
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
Proteolytic activation of the epithelial sodium channel (ENaC) heterologously expressed in Xenopus laevis oocytes can be demonstrated by combining current measurements with a biotinylation approach to investigate the appearance of ion channel cleavage products at the cell surface. Functionally important cleavage sites can be identified by using site-directed mutagenesis.