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면역학 및 감염병학

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静电粘附实验对整合素激活的T细胞研究

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

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静电粘附实验对整合素激活的T细胞研究

DOI:

10.3791/51646-v

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09:14 min

•

June 13, 2014

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Marianne Strazza, Inbar Azoulay-Alfaguter, Ariel Pedoeem, Adam Mor²

¹Department of Medicine,New York University School of Medicine, ²Departments of Pathology,New York University School of Medicine

Chapters

00:05Title
01:27Microplate and T Cell Preparation
03:57Cell Stimulation
05:43Percent Adherent Cells Analysis
07:11Results: Representative Stimulated T Cell Data
08:27Conclusion

Summary

자동 번역

静态粘合试验是一种可以用于建模的T淋巴细胞和其他细胞类型之间的相互作用的有力工具。通过标记的T细胞注入到井中涂有粘附分子，而读板器是用于以下串行洗涤量化贴壁细胞的数量所产生的相互作用。

Tags

Keywords: T Lymphocyte Adhesion Integrin Activation LFA-1 ICAM-1 Static Adhesion Assay Inside-out Signaling Cell Adhesion Cell Migration Immunological Synapse VLA-4 Fibronectin

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