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In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
 

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Article DOI: 10.3791/51683-v • 12:54 min • September 4th, 2014
September 4th, 2014 •
1, 2, 2
1Department of Life Science and Facility for Imaging by Light Microscopy, Imperial College London, 2Department of Life Sciences, Imperial College London

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The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.

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In Vivo 4-Dimensional Tracking Hematopoietic Stem Cells Progenitor Cells Adult Mouse Calvarial Bone Marrow Quiescence Proliferation Self-renewal Differentiation Blood Cell Lineages Bone Marrow Microenvironment Specimen Holder Animal Positioning Confocal Imaging Two-photon Imaging High-resolution 3D Stacks HSPCs Time-lapse Imaging Ex Vivo Labeling Movement Of HSPCs Reciprocal Interactions GFP Positive Osteoblastic Cells
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