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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
 

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Article DOI: 10.3791/51805-v 12:40 min October 20th, 2014
October 20th, 2014

챕터

요약

Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.

Tags

Keywords: Clathrin-mediated Endocytosis G Protein-coupled Receptors TIRF Microscopy Single-event Resolution Endocytic Machinery Clathrin-coated Pits Cargo Composition Fluorescence Probes Real-time Visualization Cell Biology
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