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结合母细胞和波动测试磁分选,以分析基因组不稳定性的过程中有丝分裂细胞衰老<em>酿酒酵母</em
JoVE 신문
생물학
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JoVE 신문 생물학
Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
DOI:

11:08 min

October 16, 2014

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Chapters

  • 00:05Title
  • 01:57Determination of Mutation Rate and Frequency
  • 02:50Biotin Labeling
  • 04:44Bead Labeling and Magnetic Sorting
  • 07:25Determination of Replicative Age
  • 09:09Results: Mutation Frequencies and Rates in Yeast
  • 10:39Conclusion

Summary

자동 번역

突变率在通过波动测试测量年轻酿酒酵母细胞被用于预测不同的复制年龄的母细胞的突变频率。磁分选和流式细胞术,然后用于测量实际的突变频率和母细胞的年龄,以确定从预测的突变频率的任何偏差。

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