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Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
 

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

Article DOI: 10.3791/51891-v • 11:50 min • September 10th, 2014
September 10th, 2014 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine

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요약
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Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures.

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Keywords: Cancer Stem Cells Chemoresistance Ovarian Cancer SKOV3 OVCA429 Cisplatin Stem Cell Markers Oct4 Nanog Prom1 (CD133) CD177 CD133 Tumor Relapse
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