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Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
 

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Article DOI: 10.3791/52259-v 08:39 min January 3rd, 2015
January 3rd, 2015

챕터

요약

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

Tags

Keywords: Ovarian Carcinoma Cancer Stem Cells CSCs Spheres Assay Multi Cell-based Assays Single Cell-based Assays In Vitro Serum-free Medium Growth Factors Sphere Formation Methylcellulose
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