Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Subcloning Plus Insertion (SPI) - A Novel…
JoVE Journal
Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biology
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
 

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Article DOI: 10.3791/52155-v • 09:03 min • January 8th, 2015
January 8th, 2015 •
1, 1, 2, *3, *1
1Department of Biochemistry, University of Leicester, 2Center for Fisheries, Environment and Aquaculture Sciences, 3ES Cell Facility, Centre for Core Biotechnology Services, University of Leicester
* These authors contributed equally

챕터

요약
Automatic Translation

Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.

Tags

Subcloning Plus Insertion SPI Recombineering Method Rapid Construction Gene Targeting Vectors Homologous Recombination Targeting Vector Escherichia Coli Cells Phage Recombinases Long Homology Sequences Selection Markers Allelic Exchange Vector Assembly Multiplex Approach Plasmid Gap Repair Genomic Sequence Bacterial Artificial Chromosome Libraries Correct Recombinants
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter