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T细胞受体删除环同时定量(TRECs)和K-删除重组删除环(KRECs)通过实时PCR
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
DOI:

14:14 min

December 06, 2014

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Chapters

  • 00:05Title
  • 02:34Preparation of Triple-insert Plasmid
  • 04:49Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS
  • 07:25Calculations
  • 11:59Results: TREC and KREC Quantification in Healthy Individuals
  • 12:54Conclusion

Summary

자동 번역

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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