Journal
/
/
टी सेल रिसेप्टर छांटना सर्किलों के एक साथ मात्रा (TRECs) और कश्मीर हटाने पुनर्संयोजन छांटना हलकों (KRECs) वास्तविक समय पीसीआर द्वारा
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
DOI:

14:14 min

December 06, 2014

, , , , , ,

Chapters

  • 00:05Title
  • 02:34Preparation of Triple-insert Plasmid
  • 04:49Standard Curve Preparation and Real Time PCR for Quantification of TRECS and KRECS
  • 07:25Calculations
  • 11:59Results: TREC and KREC Quantification in Healthy Individuals
  • 12:54Conclusion

Summary

자동 번역

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

Related Videos

Read Article