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Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate
 

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

Article DOI: 10.3791/52189-v • 08:49 min • December 19th, 2014
December 19th, 2014 •
1, 1
1Department of Biology, Case Western Reserve University

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Here, we present a protocol to transform flax using Agrobacterium-mediated plant transformation via floral-dip. This protocol is simple to perform and inexpensive, yet yields a higher transformation rate than the current available methods for flax transformation.

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Keywords: Floral-dip Transformation Linum Usitatissimum Agrobacterium-mediated Transformation Binary Vector Plasmid Linum Insertion Sequence (LIS-1) High Transformation Rate T1 Seeds Direct PCR Positive Transformants
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