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Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
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Chapters
- 00:05Title
- 01:00Phagocytosis Assay Technique
- 03:00Sample Analysis
- 04:43Results: Representative Activated Granulocyte Subsets Images
- 05:44Conclusion
This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity.
