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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
 

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Article DOI: 10.3791/52255-v • 08:38 min • March 3rd, 2015
March 3rd, 2015 •
*1, *1, 1, 1, 1, 1
1Département de Biologie, Institut de biologie intégrative et des systémes & PROTEO, Université Laval
* These authors contributed equally

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요약
Automatic Translation

Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

Tags

Protein-protein Interaction Screening Protein-fragment Complementation Assay PCA Living Cells Cellular Processes Protein Function Protein Regulation Protein Localization Yeast Protein-fragment Complementation Assay Direct Associations Proximal Associations Dihydrofolate Reductase (DHFR) Protein Fragment Drug Methotrexate (MTX) Differential Fitness High-density Colony Arrays Interacting Bait-prey Pairs High-throughput Protocol Robotic Platform Survival Assay Protein-protein Interactions (PPIs) Bait Proteins Endogenous Promoters
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