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Chapters
- 00:05Title
- 01:25RNA and DNA Isolation from an Enriched Astrocytic Population using FACS
- 04:03Assessing DNA Methylation Status of a Gene Using Methylation-sensitive High Resolution Melt Analysis
- 05:26Assessing Hyper-methylated Promoter Activity using a Luciferase Assay
- 08:50Results: DNA Methylation Analysis of KCNJ10 in Astrocytes
- 10:42Conclusion
DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.
