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RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
 

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Article DOI: 10.3791/52510-v • 07:01 min • April 11th, 2015
April 11th, 2015 •
*1,2, *1,2, 1,2
1Department of Psychiatry and Neurosciences, Faculty of Medicine, Université Laval, 2Centre de recherche de l'Institut universitaire en santé mentale de Québec
* These authors contributed equally

챕터

요약
Automatic Translation

Gene expression analysis of a subset of cells in a specific tissue represents a major challenge. This article describes how to isolate high-quality total RNA from a specific cell population by combining a rapid immunolabeling method with laser capture microdissection.

Tags

Keywords: Laser Capture Microdissection LCM RNA Isolation Immunolabeling Cell-specific Subpopulations Dopaminergic Cells Tissue Sectioning RNA Integrity RNA-sequencing Microarray QRT-PCR
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