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A Time Differential kleuringstechniek combinatie met Full Bilaterale Gill denervatie te Ionocytes Studie in Fish
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A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
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A Time Differential kleuringstechniek combinatie met Full Bilaterale Gill denervatie te Ionocytes Studie in Fish

DOI:
10.3791/52548-v

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11:24 min

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March 19, 2015

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1Department of Biology,University of Ottawa

Chapters

  • 00:05Title
  • 01:30Time Differential Staining Technique: Mitochondria-specific Dye Bath
  • 02:44Full Bilateral Denervation Procedure
  • 05:31Time Differential Staining Technique: Immunohistochemistry
  • 08:26Mounting and Imaging
  • 09:37Results: Distribution and Innervation of Ionocytes on a Single Filament of the First Gill Arch of Goldfish Acclimated to 25 oC
  • 10:55Conclusion

Summary

자동 번역

자동 번역

This manuscript describes a protocol to track the re-distribution of branchial ionocytes and their innervation using a time differential staining technique coupled with full bilateral gill denervation.

Tags

Keywords: Gill IonocytesTime Differential StainingFull Bilateral Gill DenervationMitochondrion-specific DyeNa+/K+ ATPaseImmunohistochemistryConfocal ImagingFish GillIonic Regulation

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