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Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
 

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Article DOI: 10.3791/52553-v • 11:25 min • April 1st, 2015
April 1st, 2015 •
1, 1, 1,2, 1, 2, 1,3
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University, 2The Biomedical Research Centre, University of British Columbia, 3Division of Neurology, Department of Medicine, Neuromuscular Disease Unit, VHHSC

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We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.

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Keywords: Bone Marrow Transplantation Myeloablative Conditioning Busulfan Chimerism Green Fluorescent Protein Central Nervous System Neurodegeneration Genetic Engineering
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