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Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
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신경과학
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Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

DOI:
10.3791/52588-v

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09:05 min

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May 06, 2015

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, , , , ,
1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen

Chapters

  • 00:05Title
  • 01:59Eye Enucleation and Isolation of Retina
  • 03:22Slice Preparation
  • 04:59Two-photon Microscopy
  • 06:14Light Stimulation and Recording
  • 06:53Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
  • 08:10Conclusion

Summary

자동 번역

자동 번역

We describe a protocol to monitor Ca2+ dynamics in the axon terminals of cone photoreceptors using an ex-vivo slice preparation of the mouse retina. This protocol allows comprehensive studies of cone Ca2+ signaling in an important mammalian model system, the mouse.

Tags

Cone PhotoreceptorsCalcium SignalingTwo-photon ImagingRetinal DegenerationTransgenic MouseTN-XL BiosensorVertical Retinal SlicesLight-evoked ResponsesAbsolute Calcium Concentrations

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