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Analyse du développement des cardiomyocytes par immunofluorescence dans le Coeur embryonnaires de souris
JoVE 신문
발생학
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JoVE 신문 발생학
Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
DOI:

10:56 min

March 26, 2015

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Chapters

  • 00:05Title
  • 01:03Snap-freezing Embryonic Mouse Hearts
  • 02:02Paraformaldehyde Fixing, Cryoprotecting, and OCT Embedding
  • 02:55Cryosectioning
  • 03:51Immunofluorescence
  • 05:16Confocal Imaging and Image Analysis
  • 08:30Results: Analysis of Cardiomyocyte Development
  • 10:27Conclusion

Summary

자동 번역

Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.

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