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Chapters
- 00:05Title
- 01:30Preparations Prior to Transfection
- 02:52Transfection
- 06:32Cell Lysate Preparation
- 07:27Dual Luciferase Assay
- 10:39Results: miRNA Targets are Identified by the 3’LIFE Assays
- 12:25Conclusion
Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.
