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Chapters
- 00:05Title
- 02:40Water Collection and Filtration
- 04:17Bacterial Concentration, DNA Extraction and Purification
- 07:52DNA Library Preparation
- 08:43Automated Gel Size Selection, Library Normalization, and Sequencing
- 11:11Representative Analysis of Size Selected DNA Libraries
- 12:51Conclusion
This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.
