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Chapters
- 00:05Title
- 00:57Parasite Synchronization and Preparation for Co-culture
- 02:27Human Peripheral Blood Mononuclear Cell (PBMC) Isolation
- 03:42Malaria/HIV Co-infection Culture and Detection of Malaria-specific Immune Responses
- 04:52Cell-specific Cytokine Production by PBMC Co-culture with P. falciparum Infected RBC
- 06:57Results: Representative IFNγ Production by Natural Killer T Cells
- 07:38Conclusion
Human co-infection is difficult to replicate in vitro. However, human malaria parasites can readily be cultured in vitro, as can freshly isolated human peripheral blood mononuclear cells naturally infected with HIV. This provides an excellent model for studying early immune responses to malaria parasites in the context of HIV co-infection.
