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从富集组织及消化细胞外基质蛋白的成肽质谱分析
JoVE 신문
생물학
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JoVE 신문 생물학
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
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从富集组织及消化细胞外基质蛋白的成肽质谱分析

DOI:
10.3791/53057-v

•

07:28 min

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July 23, 2015

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, ,
1Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Proteomics Platform,Broad Institute

Chapters

  • 00:05Title
  • 01:13Decellularization of the Tissue
  • 04:27Digestion of the ECM Proteins into Peptides
  • 06:11Results: Analysis of the Decellularization Process by Western Blot
  • 07:03Conclusion

Summary

자동 번역

자동 번역

This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

Tags

Keywords: Extracellular MatrixECMDigestionPeptidesMass SpectrometryDecellularizationEnrichmentCross-linked ProteinsGlycosylationBiomarkersDiagnosticTherapeutic Targets

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